Analysis Note
ControlWidely expressed
Application
Flow cytometry - Use 10 µL direct from the vial per 100 µL of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µL buffer.
Optimal working concentrations must be determined by the end user.
Research Sub CategoryImmunoglobulins & Immunology
Research CategoryInflammation & Immunology
This Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate is validated for use in FC for the detection of HLA-B7.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a membrane-anchored heavy chain and a light chain (beta-2 microglobulin). HLA class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells. HLA-B7 is an HLA-B serotype. B7, previously HL-A7, is a serotype that identifies the more common HLA-B*07 gene products and was one of the first ′HL-A′ antigens recognized. B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.
BIOCHEMISTRY: p I = 6.3
Immunogen
Papain solubilized HLA-B7 antigen1
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.
Specificity
Binds to the HLA-B7 antigen. It does not bind to HLA-B27 or other related HLA antigens.
Cell type Reactivity
Homozygous HLA-B7 ++
Heterozygous HLA-B7 +
Heterozygous HLA-B27 -
Non-HLA-B7, B27 -
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product has met the following criteria to qualify for the following awards: