Analysis Note

ControlWidely expressed

Application

Flow cytometry - Use 10 µL direct from the vial per 100 µL of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µL buffer.

Optimal working concentrations must be determined by the end user.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Anti-HLA-B7 Antibody, clone BB7.1, PE Conjugate is validated for use in FC for the detection of HLA-B7.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a membrane-anchored heavy chain and a light chain (beta-2 microglobulin). HLA class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells. HLA-B7 is an HLA-B serotype. B7, previously HL-A7, is a serotype that identifies the more common HLA-B*07 gene products and was one of the first ′HL-A′ antigens recognized. B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.

BIOCHEMISTRY: p I = 6.3

Immunogen

Papain solubilized HLA-B7 antigen1

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.

Specificity

Binds to the HLA-B7 antigen. It does not bind to HLA-B27 or other related HLA antigens.

Cell type Reactivity
Homozygous HLA-B7 ++
Heterozygous HLA-B7 +
Heterozygous HLA-B27 -
Non-HLA-B7, B27 -

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.